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Vitamin D, B12 & Calcium (Air India)

Vitamin D, B12 & Calcium (Air India) near me in Bangalore Rural

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The Vitamin D, B12 & Calcium (Air India) test is tailored for Air India employees to assess the levels of Vitamin D (25-OH), Vitamin B12, and Calcium in the body. These tests are essential for evaluating bone strength, nerve function and overall health. The Vitamin D, B12 & Calcium (Air India) is done as part of routine health checkup or when there are symptoms suggestive of deficiencies (like fatigue, muscle weakness, bone pain, numbness, or poor immune function), or overload (like kidney stones or nerve damage). Regular testing is crucial for maintaining optimal health and preventing long-term complications, particularly for individuals with dietary restrictions, bone health concerns, or symptoms linked to nutrient imbalances.

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What does Vitamin D, B12 & Calcium (Air India) measure?

Contains 3 tests

The Serum Calcium test measures the calcium level in your blood. Most of your body’s calcium (about 99%) is stored in bones and teeth to keep them strong. The rest (1%) circulates in the blood and helps your muscles move, nerves work, blood to clot, and heart to keep a steady rhythm.

Calcium in blood comes in two forms:

  • Bound calcium, attached to proteins like albumin
  • Ionized (free) calcium, which is active and helps many body functions

This test measures total calcium (both bound and ionized). This test cannot show if you have enough calcium in your diet. It also cannot detect bone diseases like osteoporosis. The body keeps blood calcium levels steady. It does this by taking calcium from bones during mild shortages.

What Do the Results Mean?

Pathological Factors 

Test Parameter

Interpretation (possible causes)

Low Levels

  • Underactive or damaged parathyroid glands (hypoparathyroidism and pseudohypoparathyroidism)

  • Chronic kidney disease with toxin buildup

  • Poor absorption of calcium or vitamin D

  • Liver problems like obstructive jaundice

  • Bone diseases such as osteomalacia and rickets

  • Some medicines, including cancer drugs, calcitonin, and certain diuretics

  • Low magnesium or protein levels in blood

High Levels

  • Overactive parathyroid glands (primary or secondary hyperparathyroidism)

  • Acute or chronic kidney failure, including after kidney transplant

  • Bone loss due to malabsorption or osteoporosis

  • Certain cancers, especially breast, lung, and kidney tumors

  • Medicines like vitamin D or A overdose, diuretics, hormone therapy (estrogen, androgen), tamoxifen, or lithium

Key: ⇧: Indicates High Levels , ⇩: Indicates Low Levels

Physiological Factors

🧒 Age: Older adults may have lower calcium levels due to reduced bone density and hormonal changes.

🚺 Gender: Women, especially after menopause, are more prone to calcium loss due to lower estrogen levels.

🫄 Pregnancy and Lactation: Higher calcium demand during these phases may lower calcium levels if intake is inadequate.

💊 Hormonal Influence: Parathyroid hormone (PTH), vitamin D, and calcitonin regulate calcium balance; any imbalance can alter calcium levels.

Lifestyle Factors

🥗 Diet: Inadequate intake of calcium-rich foods can lower calcium levels.

🍷 Alcohol Consumption: Excessive alcohol interferes with calcium absorption.

🚬 Smoking: Smoking may affect bone metabolism and calcium utilization.

☀️ Sun Exposure: Insufficient sunlight can reduce vitamin D levels, affecting calcium absorption.

Disclaimer: This information is for educational purposes only and should not replace professional medical advice, diagnosis, or treatment. Always consult your healthcare provider for personalized guidance.

What Do Normal Calcium Levels Mean?

Normal results indicate that your calcium metabolism is functioning properly, supporting bone health, nerve transmission, and muscle contraction.

What Do High Levels of Calcium Indicate?

High calcium levels (hypercalcemia) may indicate:

  • Overactivity of the parathyroid glands
  • Excessive vitamin D intake
  • Underlying health issues, such as certain cancers or bone disorders. 

Your doctor may recommend further tests to identify the underlying cause.

What to Do if Your Calcium Is Higher Than Normal?

If your calcium levels are higher than normal, do not self-medicate or stop supplements abruptly. Your doctor will check your health, medications, and history before deciding what to do next. In most cases, adjusting supplements or treating the root cause resolves the issue.

What Do Low Levels of Calcium Indicate?

Low calcium levels (hypocalcemia) may indicate:

  • Nutritional deficiencies,
  • Kidney or parathyroid dysfunction
  • Poor vitamin D absorption

Treatment involves calcium or vitamin D supplementation and dietary adjustments under medical supervision.

Can Calcium Results Be Inaccurate?

Yes. The Serum Calcium test results can sometimes be inaccurate. Factors that may affect the results include recent intake of calcium or vitamin D supplements, dehydration, pregnancy, hormonal therapy, and certain medications like diuretics or anticonvulsants. Minor variations in how the lab handles the sample may also impact accuracy. It is important to share your medical history and current medicines with your doctor to get a clear understanding of your calcium levels.

What Factors Can Affect Serum Calcium Results?

Though it is a reliable test, some circumstances can influence accuracy:

  • Intake of calcium/vitamin D supplements
  • Kidney or parathyroid diseases
  • Medications like lithium or diuretics
  • Prolonged immobilization
  • Hormonal imbalances
  • Sample contamination with EDTA causes falsely low calcium
  • Use plain or serum separator tubes; avoid EDTA/oxalate/citrate tubes
  • Prolonged tourniquet application (>1 min) affects results
  • Fasting sample preferred but not mandatory (8–12 hours)
  • Transport the sample at room temperature; analyze ionized calcium quickly
  • Low albumin affects total calcium; corrected or ionized calcium is preferred in specific cases (ICU, CKD, pregnancy)

What Follow-Up Tests May Be Required?

Based on your Calcium test results, your doctor may recommend additional tests to determine the cause of abnormal calcium levels and evaluate bone, kidney, or parathyroid health. These tests may include:

Table: Tests Commonly Ordered Alongside Serum Calcium Test

Test Name

Primary Purpose

Ionized Calcium Test

Measures the active (free) form of calcium 

Urine Calcium Test

Evaluates calcium loss through urine 

Vitamin D Test

Checks for vitamin D deficiency

Parathyroid Hormone (PTH) Test

Checks parathyroid gland function

Electrolyte Panel

Assesses minerals affecting calcium 

Kidney Function Test (KFT) with Electrolytes

Assesses kidney function and electrolyte balance 

Know more about Serum Calcium

The Vitamin B12 measures the amount of vitamin B12 in your blood. Vitamin B12 is important for a healthy nervous system, making red blood cells, and creating DNA in your cells. Low B12 levels are common in older adults, children, vegans or vegetarians, people with diabetes, those who had gastric bypass surgery, breastfeeding women, and people with conditions like Crohn’s disease that affect vitamin absorption. High B12 levels can occur in liver diseases, certain blood disorders, or from taking vitamin supplements. Usually, high levels from supplements are not harmful, as extra B12 is removed through urine.

What Do the Results Mean?

Pathological Factors 

Test Parameter

Interpretation (possible causes)

Low levels

  • Anaemia

  • Term pregnancy

  • Intrinsic factor deficiency

  • Partial gastrectomy/ileal damage

  • Celiac disease

  • Parasitic infestation

  • Pancreatic deficiency

  • Treated epilepsy

  • Hemodialysis 

High levels

  • Renal failure

  • Hepatocellular disorders

  • Myeloproliferative disorders and at times with excess

  • Supplementation of vitamin pills.

Physiological Factors

🧒 Age: Older adults may absorb less vitamin B12 due to lower stomach acid.

🫄 Pregnancy: Higher nutritional needs during pregnancy can temporarily lower B12 levels.

💊 Hormones: Hormone therapy or birth control pills can slightly affect B12 absorption.

Lifestyle Factors

🥗 Diet: Vegetarian or vegan diets without animal products may lead to low B12 intake.

🍷 Alcohol: Excessive alcohol can reduce B12 absorption and storage in the liver.

🚬 Smoking: Smoking cigarettes may reduce vitamin B12 activity.

Disclaimer: This information is for educational purposes only and should not replace professional medical advice, diagnosis, or treatment. Always consult your healthcare provider for personalized guidance.

What Do Normal Vitamin B12 Levels Mean?

Normal B12 levels show that your body has enough vitamin B12 for healthy red blood cells, nerve function, and metabolism. This helps maintain energy, mood, and brain health.

What Do High Vitamin B12 Levels Mean? 

High B12 levels are uncommon. They can happen due to too many supplements or health conditions like liver or kidney problems, or certain blood disorders. Your doctor may order extra tests to find the cause.

What to Do If Your Vitamin B12 Is High? 

Do not stop supplements suddenly. Your doctor will review your medical history, medications, and may suggest tests to check liver, kidney, or blood health. Often, adjusting supplements is enough to bring levels back to normal.

What Do Low Levels of Vitamin B12 Indicate?

Low vitamin B12 levels suggest a deficiency, which can lead to anemia, fatigue, and nerve damage if left untreated. Common causes include:

  • Poor dietary intake (especially in vegetarians or vegans)
  • Malabsorption due to gastric or intestinal issues
  • Pernicious anemia
  • Certain medications (e.g., metformin, proton pump inhibitors)
  • Treatment usually involves vitamin B12 supplements or injections based on deficiency severity.

Can Vitamin B12 Results Be Inaccurate?

Yes. While the test is reliable, results may sometimes be inaccurate due to recent vitamin B12 injections or supplements, pregnancy or hormonal therapy, liver or kidney diseases affecting vitamin metabolism, or laboratory variations in sample handling.

What Factors Can Affect Vitamin B12 Results?

Though it is a reliable test, several factors can influence accuracy:

  • Recent intake of vitamin B12 supplements or injections
  • Liver or kidney disorders
  • Alcohol consumption or chronic illness
  • Use of medications like metformin or oral contraceptives
  • Pregnancy or hormonal therapy

What Follow-Up Tests May Be Required?

Based on your Vitamin B12 results, your doctor may recommend additional tests to get a clearer picture of your health or to monitor possible complications. These may include: 

Table: Tests Commonly Ordered Alongside Vitamin B12

Test Name

Primary Purpose

Folate (Vitamin B9) Test

Check folate levels, since low folate can also cause anemia similar to B12 deficiency.

Homocysteine Test

Measure homocysteine in the blood, which can rise when B12 is low.

Methylmalonic Acid (MMA) Test

Detect early B12 deficiency by measuring MMA, which increases when B12 is low.

Complete Blood Count (CBC) Test

Provide details about red blood cells and hemoglobin to indicate type of anemia.

Intrinsic Factor Antibody Test

Detects autoimmune causes of B12 deficiency.

Gastrointestinal Absorption Tests

Checks if the stomach or intestines are absorbing B12 properly, e.g., after gastric surgery or in conditions like Crohn’s disease.

How Does the Vitamin B12 Test Differ from Other Blood Tests?

It is important to understand how the Vitamin B12 test differs from other common blood tests:

Complete Blood Count (CBC): Checks overall blood health and red blood cells. CBC can suggest anemia but doesn’t show the cause. Vitamin B12 specifically measures the nutrient needed to produce healthy red blood cells.

Folate (Vitamin B9) Test: Folate levels also affect red blood cell production. Unlike B12, folate deficiency alone does not directly indicate nerve or brain-related issues.

Iron Studies: Tests like serum iron or ferritin detect iron-related anemia. Vitamin B12 deficiency anemia occurs even if iron levels are normal.

Homocysteine & Methylmalonic Acid (MMA): These are functional tests that rise when B12 is low. The Vitamin B12 test directly measures the B12 level, making it the first step to detect deficiency.

Know more about Vitamin B12

The Vitamin D (25-Hydroxy) test measures the levels of vitamin D in the body. Vitamin D is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. It exists in two forms: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D2 is present in plants, such as yeast or mushrooms, and is available as a supplement in fortified foods. Vitamin D3 is found in foods like cheese, green vegetables, mushrooms, egg yolks, and fatty fish.

Both forms of vitamin D need to be converted in the liver and kidneys before the body can use them effectively. This test shows the total level of vitamin D (D2 + D3) circulating in the body and guides your doctor in recommending the right treatment or lifestyle changes if needed.

What Do the Results Mean?

Pathological Factors 

Test Parameter

Interpretation (possible causes)

Normal / Optimal

  • Sufficiency

Possible causes: HPT (Hyperparathyroidism)

Humoral hypercalcemia of malignancy

Low

  • Deficiency 

Possible causes can be renal failure, hyperphosphatemia, vitamin D–dependent rickets, types 1 and 2

Borderline / Moderate Low

  • Insufficiency (due to inadequate dietary intake or low sunlight exposure)

High / Excess

  • Hypervitaminosis

Possible causes can be sarcoidosis, non-Hodgkin lymphoma 

Key: ⇧: Indicates High Levels , ⇩: Indicates Low Levels

Physiological Factors

🧒 Age: Older adults synthesize less vitamin D from sunlight primarily because of age-related changes in their skin.

🫄 Pregnancy: Vitamin D demand increases during pregnancy for fetal bone development.

💊 Hormonal Influence: Hormonal changes or contraceptive use may alter vitamin D metabolism.

Lifestyle Factors

🌤️ Sun Exposure: Limited sunlight reduces vitamin D synthesis.

🥗 Diet: Low intake of vitamin D-rich foods (like fish, egg yolks, or fortified dairy) can cause deficiency.

🍷 Alcohol Consumption: Chronic alcohol use affects liver function, impacting vitamin D metabolism.

🚬 Smoking: Smoking is closely associated with reduced bone density and altered vitamin D activity.

Disclaimer: This information is for educational purposes only and should not replace professional medical advice, diagnosis, or treatment. Always consult your healthcare provider for personalized guidance.

What Do Normal Vitamin D (25-Hydroxy) Levels Mean?

Normal vitamin D levels indicate your body has sufficient vitamin D for bone health, muscle strength, and immune function.

What Do High Levels of Vitamin D (25-Hydroxy) Indicate?

High vitamin D levels may occur due to excessive supplementation or conditions like sarcoidosis or hyperparathyroidism. Persistently high levels can cause vitamin D toxicity, leading to high blood calcium (hypercalcemia), which may result in nausea, vomiting, muscle weakness, and, in severe cases, kidney stones or kidney damage.

What to Do if Your Vitamin D (25-Hydroxy) Is Higher Than Normal?

If your Vitamin D (25-Hydroxy) level is high, consult your doctor for further guidance. They may review your dosage, identify causes of high calcium like excess supplement intake, or check for conditions such as hypercalcemia. Do not stop or adjust supplements without your doctor’s advice.

What Do Low Levels of Vitamin D (25-Hydroxy) Indicate?

Low levels suggest deficiency, which may cause weak bones, muscle pain, or fatigue. Common causes include:

  • Lack of sunlight exposure
  • Poor dietary intake
  • Malabsorption syndromes (e.g., celiac disease)
  • Obesity or liver/kidney disease

Treatment involves lifestyle changes, dietary improvement, or vitamin D supplements.

Can Vitamin D (25-Hydroxy) Results Be Inaccurate?

Yes. Results may vary due to recent supplement intake, laboratory variations, or conditions affecting vitamin D metabolism, such as liver or kidney disease. In some cases, additional tests may be advised to confirm the deficiency.

What Factors Can Affect Vitamin D (25-Hydroxy) Results?

Though it is a reliable test, some circumstances can influence accuracy:

  • Recent vitamin D or calcium supplement intake
  • Limited sun exposure
  • Chronic illness or liver/kidney disorders
  • Malabsorption syndromes
  • Obesity or hormonal imbalances

Know more about Vitamin D (25-Hydroxy)

Vitamin D, B12 & Calcium (Air India) test price for other cities

Price inMumbaiRs. 779
Price inNew DelhiRs. 779
Price inChennaiRs. 779
Price inGhaziabadRs. 779
Price inHyderabadRs. 779

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Package contains 3 tests
Serum Calcium
Vitamin B12
Vitamin D (25-Hydroxy)