Accurex Accudx CQ IL-6 Fast Test Kit

Accurex Accudx CQ IL-6 Fast Test Kit is used for the determination of Interleukin 6 (IL-6) in human serum, plasma, whole blood, and peripheral blood samples. IL-6 is an early marker in acute inflammation and this test can be used as an aid in inflammatory diseases. The lest uses an anti-human IL-6 monoclonal antibody I conflated with fluorescence latex coated on the junction of nitrocellulose membrane and sample pad, and another anti-human IL-6 monoclonal antibody I coated on the test line. After the sample has been applied to the test strip, the fluorescence latelabelled anti-human IL-6 antibody I bind with the IL-6 in the sample and forms marked antigen-antibody complex is captured on the last line by anti-human IL-6 antibody Il. The fluorescence intensity of the test increases in proportion to the amount of IL-6 in a sample. Then insert the last card into AccuDx CQ Immunofluorescence Quantitative Analyzer/automatically inserted by AccuDx CO Immunofluorescence Quantitative Analyzer (hereafter referred to as AccuDx CO). the concentration of IL-6 in a sample will be measured and displayed on the screen. The value will be stored in AccuDx CQ and available for downloading. The result can be easily transmitted to the laboratory or hospital information system.

Uses:
IL-s Fast Test Kit (Immunofluorescence Assay is intended for in vitro quantitative determination of interleukin 6 (IL-6) in human serum, plasma, whole blood and peripheral blood samples. IL-6 is an early marker in acute inflammation and this test can be used as an aid in inflammatory diseases.

Product Specifications and Features:

• IL-6 is a cytokine that functions in the inflammation and maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response

• This classical responsiveness to IL-6 is governed by a receptor complex consisting of two membrane-bound subunits, an 80 Da cognate Alpha-chain (IL-6R Alpha), and a ubiquitously expressed 130-KDa Beta-chain receptor (gp130) which acts as the universal signal transducing element for all IL-6 family cytokines

• It has many different cells that are capable of IL-6 synthesis including monocytes/macrophages, and fibroblasts. endothelial cells, keratinocytes, and mast cells. T cells and many tumour cell lines

• In vivo and in vitro, IL-6 acts as a differentiation factor for B cells and an activation factor for T cells [L-6 is a patent growth factor of different human myelomas and is active in a concentration less than 10 pg/mL. IL-3 and IL-6 show in vitro synergistic effects in the differentiation of hematopoietic progenitor cells

• Elevated IL-6 serum or plasma levels may occur in different diseases including sepsis, autoimmune diseases, lymphomas, AIDS, lonely liver disease and in patients with infections, or transplant rejection

Directions For Use:
Test Procedure:

• Collect specimens according to the user manual

• Test card, sample, and reagent should be brought to room temperature before testing

• Confirm SD card lot No. following test kit lot No

• Perform "SD card' calibration when necessary

• Enter testing interface of AccuDx CQ

• Remove the test card from the sealed pouch immediately before use

• Label the test card with patient or control identification

• Put the test card on a clean table, horizontally placed

• Using a sample transfer pipette, deliver 100 L of the sample into the sample port on the test card (for a whole blood sample, one drop of whole blood butter must be added after loading 100 L on the test card)

• Reaction time is 15 minutes, insert the test card into AccuDx CO and start the test after the reaction time is elapsed. The result will be shown on the screen and printed automatically

Safety Information:

• For in vitro diagnostic use only

• Do not use the kit beyond the expiration date

• Do not use the test card if the foil pouch is damaged

• Do not open pouches until ready to perform the test

• Do not reuse the test card

• Do not reuse the pipel

• Handle all specimens as potentially infectious

• Proper handling and disposal methods should be followed following local regulations

• Carefully read and follow the user manual to ensure proper test performance