How can we help?

Home

FSH-LH-Testosterone

Also referred as

Hormonal profile

Hormonal panel

532+ booked recently

For men & women

Earliest reports in

18 hours

Contains

3 tests

Know more about this test

An FSH-LH-Testosterone test helps assess hormonal balance and function in both men and women. These hormones play an important role in reproductive health, with Follicle-Stimulating Hormone (FSH) and Luteinizing Hormone (LH) regulating the menstrual cycle in women and sperm production in men, while testosterone is primarily responsible for men's sexual characteristics and fertility.

Samples required

Blood

Find out

Why is this test booked?

Preparation for this test

Sample Collection

Who will collect your samples?

Understanding FSH-LH-Testosterone

An FSH-LH-Testosterone test evaluates hormonal balance in the realm of reproductive health. It assesses three key hormones-Follicle-Stimulating Hormone (FSH), Luteinizing Hormone (LH), and testosterone. These hormones, produced by the pituitary gland and gonads (testes in men and ovaries in women), play pivotal roles in various physiological processes, important for reproductive and overall well-being.

In women, FSH and LH help regulate the menstrual cycle. FSH helps eggs in the ovaries to grow, while LH triggers the release of mature eggs during ovulation. LH also helps produce progesterone, a hormone essential for maintaining pregnancy. Monitoring FSH and LH levels tells about ovarian function, menstrual irregularities, and fertility. On the other hand, in men, FSH and LH are important for sperm production. FSH helps in the growth of sperm-producing structures, while LH stimulates testosterone production. Testosterone is a key hormone for the development of secondary sexual characteristics like deepening of voice, muscle growth, and facial and body hair in men. Also, this hormone helps maintain sexual desire, bone health, and overall vitality in men.

An FSH-LH-Testosterone test is commonly suggested by a doctor to diagnose and monitor various reproductive disorders, such as polycystic ovary syndrome (PCOS), hypogonadism (low testosterone levels), and menstrual irregularities. By assessing the levels of FSH, LH, and testosterone, doctors can identify hormonal imbalances, guide treatment decisions, and monitor the effectiveness of interventions aimed at restoring hormonal balance and optimizing reproductive health.

Usually, no special preparation is needed for an FSH-LH-Testosterone test. However, in women, this test is advised on the second or third day of the menstrual cycle whereas for men, it can be done at any time. Additionally, you may discuss it with your doctor to temporarily stop taking certain medications or supplements, such as birth control pills, etc. as these may influence the test results.

Lab test results may vary across different laboratories based on the different methodologies. Talk to your doctor about your specific test results. The results will help them evaluate your medical condition; make recommendations for diet, exercise, or medication; and formulate an overall treatment plan based on your condition.

What does FSH-LH-Testosterone measure?

Contains 3 tests

An FSH-LH-Testosterone test measures the levels of three key hormones - follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone in the blood. These hormones are vital for hormonal function and overall reproductive health in both men and women.

In females, FSH stimulates the growth and recruitment of immature ovarian follicles in the ovary. When the follicle matures and reaches 8–10 mm in diameter it starts to secrete significant amounts of estradiol. The sharp increase in estradiol production by the dominant follicle causes an LH surge. LH levels rise just before ovulation to trigger the release of a mature egg. This way, FSH levels help assess ovarian function in women. In males, FSH levels help assess testicular function. It helps in the growth of sperm-producing structures, while LH stimulates testosterone production.

Testosterone plays a key role in the development of male reproductive tissues such as testicles and prostate, as well as promoting secondary sexual characteristics such as increased muscle and bone mass, and the growth of body hair.

The levels of these hormones are typically low during younger ages. However, as adolescence approaches, the brain starts producing gonadotropin-releasing hormone (GnRH). GnRH stimulates the pituitary gland to release FSH and LH. These gonadotropin hormones play a crucial role in the production of sex hormones such as testosterone, which are responsible for initiating the changes of puberty and controlling reproductive function in both men and women.

Luteinising Hormone

The Luteinising Hormone test determines the level of luteinizing hormone that promotes the functioning of the gonads, i.e., testes in men and ovaries in women. LH is produced by the pituitary gland, a pea-sized gland situated at the base of the brain. The production and release of LH are controlled by a system known as the hypothalamic-pituitary-gonadal axis. A hormone called GnRH is released from the hypothalamus of the brain and binds to the cell receptors in the anterior pituitary gland, which in turn prompts the synthesis and release of luteinizing hormone. LH then travels across the blood and attaches to its target cells in the testes in men and ovaries in women to facilitate optimal sexual and reproductive activities.

Know more about Luteinising Hormone

Follicle Stimulating Hormone

The Follicle Stimulating Hormone test measures the level of FSH in your body. FSH is produced and subsequently released into the blood by the pituitary gland. Its production is controlled by a feedback mechanism involving a hormone situated in the hypothalamus in the brain. The released FSH travels through your blood and then attaches to receptors in the ovaries and testes. It exerts functional effects on the reproductive organs, such as sexual development, puberty, and maturation. FSH levels vary throughout the menstrual cycle in response to estradiol and progesterone. Any disruption or issue in this feedback mechanism can prevent the normal function of the testicles or ovaries. Thus, determining its level provides crucial information regarding your health.

Know more about Follicle Stimulating Hormone

Testosterone Total

The Testosterone Total test measures the level of testosterone hormone available in the blood. This includes two forms of testosterone: free testosterone and bound testosterone. Bound testosterone is attached to proteins, mainly to sex hormone-binding globulin (SHBG) and albumin. It cannot enter the body's cells or directly affect tissues and organs. Instead, it acts as a store of testosterone in the blood and can be released when the body needs it. Free testosterone is not attached to any proteins and is the active form. It can easily transport to various tissues, and affects key functions like sex drive, muscle growth, bone strength, and energy levels.

Know more about Testosterone Total

Frequently booked together

FAQs related to FSH-LH-Testosterone

Why is an FSH-LH-Testosterone test done?

An FSH-LH-Testosterone test is done to assess the levels of hormones- FSH, LH, and testosterone that are essential for understanding reproductive health and fertility in both men and women.

Is there any risk associated with an FSH-LH-Testosterone test?

There is usually no risk associated with this test. However, some people may feel a slight sting when a needle is pricked to collect a blood sample and may experience some bleeding that is normal and usually not bothersome.

What factors can affect the levels of FSH, LH & testosterone?

Factors such as stress, physical activity, medications, and underlying health conditions can affect FSH, LH, and testosterone levels

What are the symptoms of hormonal imbalance in women?

Women with hormonal imbalances often experience heavy or irregular periods, mood swings, raised blood sugar levels, bloating, fatigue, excessive facial hair growth, and unexplained weight changes.

What are the symptoms of hormonal imbalance in men?

Men with hormonal imbalances may experience hair thinning, hair loss, weight gain/loss, low sex drive, erectile dysfunction, fatigue, and depression, and show poor semen parameters, including low sperm count, motility, and morphology, leading to infertility.

Can levels of FSH vary during different days of the menstrual cycle?

Yes, FSH levels in women change throughout the menstrual cycle. Its levels peak during the menstruation and ovulatory phase and drop down during the late follicular and luteal phases of the menstrual cycle.

What happens if the test results show high FSH levels in women?

High levels of FSH in women can indicate loss of ovarian function or ovarian failure, menopause, polycystic ovarian syndrome, chromosomal abnormalities (e.g., Turner's syndrome), and reduction in the production of good-quality eggs and embryos for fertilization.

What are the causes of high levels of FSH in men?

High levels of FSH in men may indicate Klinefelter’s syndrome, absence of testicles or impaired functioning of testicles, damage to testicles by habits like alcohol dependence, or treatments such as X-rays or chemotherapy.

What happens when LH levels are high?

In females, the rise in LH triggers ovulation and also affects progesterone levels. However, too high LH is associated with polycystic ovary syndrome and infertility. Further, high levels of LH cause early puberty, poor sperm morphology as well as motility, and indicate damaged testicles in males.

What happens when LH levels are low?

Low LH levels can signify that your pituitary gland is not making sufficient LH which is needed to induce changes in your body that support sexual development or reproduction.

What are the signs of low testosterone levels in men?

The signs of low testosterone levels in men include decreased muscle mass, lack of body and facial hair, very small testicles, and development of breast tissue.

Will taking supplements help to raise testosterone levels?

While some supplements claim to boost testosterone levels, it's important to approach them with caution and should be taken after consulting your medical practitioner. The body regulates hormone levels, and altering them without medical advice may lead to unintended consequences. Before considering supplements, consult your doctor to ensure they are suitable and safe for your individual health condition.

Content created by

Dr. Swati Gupta (Anand)

MBBS, MD Lab Medicine

Want to know moreRead our editorial policy

Last Updated

7th Nov 2025