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Vitamin D (25-Hydroxy) & Vitamin B12

Also referred as
25-OH vitamin D
+4
Calcidiol
Cyanocobalamin
Methylcobalamin
Cobalamin
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Contains
2 tests
Test price:
₹1149
₹2750
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The Vitamin D (25-Hydroxy) & Vitamin B12 test measures the level of vitamin D and vitamin B12 in your body. This test helps determine deficiencies related to vitamin D and B12. It also helps assess if you have too much of these vitamins in your body (most likely from excessive supplements). Vitamin D helps keep bones, teeth, and muscles healthy, whereas vitamin B12 maintains blood, brain, and nerve functions.

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Blood
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No special preparation is required.
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TATA 1mg Labs is a cutting-edge facility delivering top-quality diagnostic services right to your doorstep. We take pride in three core values: Assured Quality, Best Prices and Timely Reports. Transparency is at the heart of our operations, ensuring our customers always have clear and reliable information. Our dedicated team is committed to offering an exceptional customer experience and continually innovates to meet and exceed customer expectations.
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Understanding Vitamin D (25-Hydroxy) & Vitamin B12

Vitamins, although required in small amounts, are significant in maintaining overall health and well-being.  Vitamin D (25-hydroxy) plays a critical role in various body functions, including:

  • Supports immune system functioning
  • Helps maintain healthy bones
  • Promotes cell growth

Vitamin D also acts as a hormone because it is synthesized in the skin (via sunlight) and then converted to its active form (known as calcitriol) in the kidneys. This active form helps regulate the levels of calcium and phosphorus in the body. Low vitamin D levels can result in an increased risk of fractures, muscle weakness and lethargy, dysfunction of the immune system, and may be associated with an increased risk of diabetes.

Vitamin B12, also known as cobalamin, belongs to the B complex group of vitamins. It is vital for various body functions such as:

  • Aids in the production of red blood cells (RBCs)
  • Supports repair and regeneration of cells and tissues
  • Promotes optimal brain function and nerve health

Some common symptoms of vitamin B12 deficiency include fatigue, mouth ulcers, loss of appetite, burning sensation in the feet, hands, arms, and legs, muscle weakness, etc.

The Vitamin D (25-Hydroxy) & Vitamin B12 checks for the levels of Vitamin D and Vitamin B12 in your body. The vitamin D test is important for assessing bone health and calcium metabolism. It helps detect deficiencies that can lead to rickets in children and osteomalacia or osteoporosis in adults. The vitamin B12 test helps determine the response to vitamin B12 therapy in conditions like anemia. Additionally, it is important to monitor parameters to maintain a healthy pregnancy. Low vitamin B12 levels have been associated with adverse pregnancy outcomes like anemia, low birth weight, and restricted fetal growth. The Vitamin D (25-Hydroxy) & Vitamin B12 test can help determine vitamin D and vitamin B12 deficiencies or if you have too much vitamin D or B12 in your body (most likely from excessive supplements). 

Usually, no special preparation is required for this test. Eat and drink as per the daily routine. However, do let the doctor know about all the medications being taken, as some of them may affect the accuracy of the test results.

Test result ranges are approximate and may differ slightly between labs depending on the methodology and laboratory guidelines. Talk to the doctor about specific test results. They can help you understand what the results mean, suggest lifestyle changes like diet or exercise, give medicine if needed, and plan the right treatment for you.

Lifestyle Tips to Support Healthy Vitamin D and Vitamin B12 Levels:

🥤 Hydration: Drink enough water daily to support proper digestion.

🌞 Exposure to the sun: Get regular morning sunlight for natural vitamin D synthesis.  

🏃 Activity (Exercise): Regular physical activity, especially outdoors, supports vitamin D production and may enhance B12 metabolism.

🍔 Lifestyle (Balanced Diet): Include vitamin D-rich foods (like eggs, mushrooms, salmon, fortified milk) and vitamin B12-rich foods (like meat, fish, dairy, or fortified cereals for vegetarians/vegans) in your diet.

👩‍⚕️ Talk to Your Doctor (Consultation): Avoid self-supplementation.  Consult your doctor for proper guidance on vitamin D or B12 levels and safe supplementation if needed.

🚭 Habits (Smoking and Alcohol): These habits can interfere with the absorption and activation of vitamins B12 and D.

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What does Vitamin D (25-Hydroxy) & Vitamin B12 measure?

Contains 2 tests

The Vitamin D (25-Hydroxy) & Vitamin B12 test is tailored to determine the levels of two important vitamins in your body: vitamin D (25-OH) and vitamin B12. Vitamin D is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. On the other hand, vitamin B12 is vital for maintaining a healthy nervous system, making red blood cells, and creating the genetic material of our cells.

This test is generally recommended as a part of routine health checkups, nutritional assessments, or to monitor vitamin levels in individuals with dietary restrictions or those who are on vitamin supplements.

The Vitamin B12 test measures the amount of vitamin B12 in your blood. Vitamin B12 is important for a healthy nervous system, making red blood cells, and helping in DNA formation. Low B12 levels are common in older adults, children, vegans or vegetarians, people with diabetes, those who had gastric bypass surgery, breastfeeding women, and people with conditions like Crohn’s disease that affect vitamin absorption. High B12 levels can occur in liver diseases, certain blood disorders, or from taking vitamin supplements. Usually, high levels from supplements are not harmful, as extra B12 is removed through urine.

Know more about Vitamin B12

The Vitamin D (25-Hydroxy) test measures the levels of vitamin D in the body. Vitamin D is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. It exists in two forms: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D2 is present in plants, such as yeast or mushrooms, and is available as a supplement in fortified foods. Vitamin D3 is found in foods like cheese, mushrooms, egg yolks, and fatty fish.

Both forms of vitamin D need to be converted in the liver and kidneys before the body can use them effectively. This test shows the total level of vitamin D (D2 + D3) circulating in the body and guides your doctor in recommending the right treatment or lifestyle changes if needed.

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Packages containing Vitamin D (25-Hydroxy) & Vitamin B12
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Vitamin D (25-Hydroxy) & Vitamin B12 test price for other cities

Price inBangaloreRs. 1149
Price inHyderabadRs. 649
Price inPuneRs. 1149
Price inMumbaiRs. 1149
Price inNew DelhiRs. 1149
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FAQs related to Vitamin D (25-Hydroxy) & Vitamin B12

The Vitamin D (25-Hydroxy) & Vitamin B12 test can be done at any time of the day as no fasting is required for getting tested or follow your doctor’s advice.
No, the Vitamin D (25-Hydroxy) & Vitamin B12 test is generally a safe procedure. While rare, bruising, bleeding, and infection at the puncture site may occur. In very uncommon cases, there can be swelling of the vein after the blood withdrawal.
Bone pain, depression, hair loss, muscle weakness, appetite loss, pale skin, and sleep disturbances are some common signs and symptoms of vitamin D deficiency.
Insufficient sunlight exposure or a vitamin D-deficient diet are the primary cause of vitamin D deficiency.
Low vitamin D can have adverse effects on the whole body. It can increase the risk of heart disease, high blood pressure, diabetes, infections, and immune system disorders.
Always refer to your doctor in case your test results indicate low levels of vitamin D. Although consuming oily fish such as salmon or sardines, red meat, egg yolks, and fortified cereals may help improve vitamin D levels. Additionally, sufficient exposure to the sun can assist you in naturally increasing your levels of vitamin D.
Muscle weakness, loss of appetite, vision problems, a painful tongue, mouth ulcers, a fast and irregular heartbeat, shortness of breath, tingling or numbness, and pins and needles or burning sensations in the feet, hands, arms, and legs are common signs and symptoms of vitamin B12 deficiency.
Deficiency of vitamin B12 could be due to poor dietary intake, pernicious anemia, and certain types of stomach-related surgeries, like weight loss surgery or intestinal surgery.  Some medicines and immune system problems like lupus or Graves' disease can also cause it. Talk to your doctor if you have any of these issues.
Low vitamin B12 levels can lead to symptoms like fatigue, weakness, pale skin, appetite loss, weight loss, infertility, numbness or tingling in hands and feet, balance issues, depression, confusion, memory problems, and mouth soreness. In infants, it may cause growth delays and anemia. Early treatment is crucial to prevent nerve damage.
Always consult your doctor if your vitamin B12 levels are lower than normal. Incorporating food like meat, fish, eggs, milk, cheese, soy products, and fortified cereals may also help boost your vitamin B12 levels.
Regular sun exposure is the most natural way to get enough vitamin D. Usually, you need sunlight for about 10 to 30 minutes several times weekly. However, darker-skinned people may need a little longer than this. Exposure time also depends on how sensitive your skin is to sunlight.
a) 0–12 months: 400 IU/day; b). Ages 1–70: 600 IU/day; c) Over 70: 800 IU/day. For adults with low levels (<30 ng/mL), 1,500–2,000 IU/day is advised to restore balance (as per Endocrine Society).
Yes, low Vitamin B12 levels can contribute to mental health problems like depression, anxiety, confusion, and memory issues. Vitamin B12, along with other B vitamins, helps control brain chemicals that affect mood and behavior.
Pregnant women need enough nutrients to help the baby grow and develop well. Low Vitamin B12 levels can lead to problems like anemia, low birth weight, and slow growth in the baby. The baby can also suffer from serious problems like incomplete brain development, and other brain-related issues. Talk to your doctor if you are pregnant or planning to conceive to know about any supplements you may need during pregnancy.
Recommended for everyone
This package is designed with everyone’s overall health considerations in mind, offering assessments to address a wide range of wellness needs.
Package can be booked by :
Men
Women
Contains 2 tests
Vitamin B12
Vitamin D (25-Hydroxy)
Report delivery
Faster Report
₹49 per patient
7 hrs
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Limited slots only, you can choose faster report option when you add the test
Standard time
No extra charges
12 hrs
For slots after 11 AM, report will be delivered in 15 hours.
Samples required
Blood
1 vial required
Our phlebotomist will draw a blood sample, typically from a vein in your inner elbow.
Preparations
1
No special preparation is required.
Why is this test booked?
1
A. In Symptomatic Individuals
2
Chronic fatigue
3
Muscle weakness or cramps
4
Numbness or tingling
5
Bone or joint pain
6
Rapid breathing or shortness of breath
7
Blurred vision
8
Susceptibility to frequent infections
9
B. In health-specific conditions
10
Osteoporosis
11
Inflammatory bowel diseases (e.g., Crohn’s disease, ulcerative colitis)
12
Malabsorption syndromes (e.g., celiac disease)
13
Chronic kidney or liver disease
14
History of gastrectomy or other gastrointestinal surgery
15
Anemia (especially megaloblastic anemia)
16
C. In Special Populations
17
Pregnant or breastfeeding women
18
Elderly individuals
19
Infants who are exclusively breastfed
20
D. In Individuals With Lifestyle-Related Risks
21
Limited sun exposure (risk for Vitamin D deficiency)
22
Strict vegan or vegetarian diet (risk for Vitamin B12 deficiency)
23
Obesity
24
Chronic alcohol consumption
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